Tools for Groundwater Protection Planning: An Example from McHenry County, Illinois, USA

/ This paper presents an approach for producing aquifer sensitivity maps from three-dimensional geologic maps, called stack-unit maps. Stack-unit maps depict the succession of geologic materials to a given depth, and aquifer sensitivity maps interpret the successions according totheir ability to transmit potential contaminants. Using McHenry County, Illinois, as a case study, stack-unit maps and an aquifer sensitivity assessment were made to help land-use planners, public health officials, consultants, developers, and the public make informed decisions regarding land use. A map of aquifer sensitivity is important for planning because the county is one of the fastest growing counties in the nation, and highly vulnerable sand and gravel aquifers occur within 6 m of ground surface over 75% of its area. The aquifer sensitivity map can provide guidance to regulators seeking optimal protection of groundwater resources where these resources are particularly vulnerable. In addition, the map can be used to help officials direct waste-disposal and industrial facilities and other sensitive land-use practices to areas where the least damage is likely to occur, thereby reducing potential future liabilities. KEY WORDS: Geologic mapping; Groundwater; Aquifers; Aquifer sensitivity; Land-use planning     

Seabird eggs as bioindicators of chemical contamination in Chile

Seabird eggs were used as bioindicators of chemical contamination in Chile. Brown-hooded Gull (Larus maculipennis), Kelp Gull (Larus dominicanus), Trudeau's Tern (Sterna trudeaui), Neotropic Cormorant (Phalacrocorax brasilianus), and Pink-footed Shearwater (Puffinus creatopus) eggs were sampled at different breeding sites during the 1990s. Mercury and organochlorines (PCBs, DDT, HCB, HCH, and PCP) were quantified to reveal the interspecific differences, spatial and temporal trends in contamination levels. Trudeau's Tern displayed the highest levels of mercury (486 ng g(-1) wet weight). The highest sumDDT concentrations were measured in Brown-hooded Gulls (726 ng g(-1)). PCB levels were similar among the species (102-236 ng g(-1)), but the composition of the PCB mixture was different in Pink-footed Shearwaters. With the exception of the Brown-hooded Gull, all species studied presented similar and low levels of organochlorines (sumOHa). Residues of PCB and related compounds were not detected in any of the seabird eggs analyzed in Chile. Geographical variation was low, although levels of industrial chemicals were slightly higher in eggs from Concepción Bay, and agricultural chemicals in eggs from Valdivia. Also interannual variation was low, but some evidence was found of decreasing levels in gull eggs throughout the time of the study. The causes of the low levels and small variability in space and time of environmental chemicals in Chilean seabirds are discussed. We propose the use of seabirds in future monitoring of the development of chemical contamination in Chile.     

Quantification of bacterial lipopolysaccharides (endotoxin) by GC-MS determination of 3-hydroxy fatty acids

A GC-MS method for the quantification of bacterial lipopolysaccharides (LPS, endotoxin) is presented. After hydrolytic cleavage of 3-hydroxy fatty acids (3-OH FAs) from the lipid A region of LPS, derivatisation of both the hydroxyl and the carboxyl group was performed in one step with a mixture of methyl-bis(trifluoracetamide)(MBTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoracetamide (MTBSTFA). Using GC-MS in the EI mode with selected ion monitoring (SIM) for analysis, baseline separation of 3-OH FAs (and of possibly interfering 2-OH FAs) was achieved. The sensitivity of the method (LOD 7-50 pg/injection for the different 3-OH FAs investigated) allows for the efficient quantification of LPS in occupational and environmental samples. Degradation of 3-OH FAs as well as of their derivatives during sample preparation and GC-MS separation as a possible source of errors in analytical methods based on 3-OH FA determination is reported for the first time. Thermal elimination of water from the underivatised 3-OH FAs and of trifluoroacetic acid from the derivatives was identified as the cause of degradation. The resulting alpha,beta-unsaturated compounds showing the same mass spectra as the 3-OH FA derivatives were detected as more or less prominent satellite peaks. By using alkaline instead of acidic hydrolysis and cool on-column instead of split/splitless injection, elimination was reduced to an acceptable level.     

Occupational Health Care in Small and Medium-Sized Enterprises—Introduction of Services to Craftsmen by Using Their Professional Networks

In order to ensure equality of occupational health care among employees of small and medium-sized enterprises (SMEs) and of larger companies, amendments in the regulations of the numerous German accident insurance funds had to be made to provide for full availability of services, as requested by German and European law. According to these amendments, sectors formerly exempted due to small size and due to lack of an adequate number of qualified personnel, had to be covered by occupational health care. In order to reach this target group new strategies of care delivery had to be developed, making use of pre-existing infrastructure and networks.

In Germany, district trade association (Kreishandwerkerschaften) have proved to be very effective for introducing occupational safety and health care into SMEs by either hiring external multidisciplinary services or by establishing a common service to be used by all associated crafts establishments. In a study conducted by the Federal Office for Health and Safety at Work in 1996 (Boldt, Gille, &Grahl, 1997), 7 district trade association were looked at in detail for their strategies. The results were discussed and supplemented in a 2-day workshop.     

Rapid tissue reduction and recovery in the sponge Aplysinella sp.

We observed a pronounced, yet reversible tissue reduction in the tropical sponge Aplysinella sp. under non-experimental conditions in its natural habitat, after transfer into seawater tanks, as well as after transplantation from deep to shallow water in the field. Tissue reduction resulted in the formation of small “reduction bodies” tightly attached to the sponge skeleton. Although volume loss and gain were substantial, both tissue reduction and regeneration were often remarkably rapid, occurring within few hours. Microscopic analysis of the reduction bodies revealed morphological similarities to previously described sponge primmorphs, with densely packed archaeocytes and spherulous cells enclosed by a thin layer of epithelial-like cells. Denaturing gradient gel electrophoresis (DGGE) revealed pronounced changes in the sponge-associated microbial community upon tissue reduction during laboratory and field experiments and following changes in ambient conditions after transplantation in the field. Generally, the microbial community associated with this sponge proved less stable, less abundant, and less diverse than those of other, previously investigated Verongid sponges. However, one single phylotype was consistently present in DGGE profiles of Aplysinella sp. This phylotype clustered with γ-proteobacterial sequences found previously in other sponge species of different taxonomic affiliations and geographic provenances, as well as in sponge larvae. No apparent changes in the total secondary metabolite content (per dry weight) occurred in Aplysinella sp. upon tissue reduction; however, comparative analysis of intact and reduced tissue suggested changes in the concentrations of two minor compounds. Besides being ecologically interesting, the tissue reduction phenomenon in Aplysinella sp. provides an experimentally manipulable system for studies on sponge/microbe symbioses. Moreover, it may prove useful as a model system to investigate molecular mechanisms of basic Metazoan traits in vivo, complementing the in vitro sponge primmorph system currently used in this context.     

Reference conditions for rivers of the German Baltic Sea catchment: reconstructing nutrient regimes using the model MONERIS

We introduce an approach for establishing reference conditions (RC) for rivers of the German Baltic Sea catchment, based on predictive modelling. An extensive data set of statistics from the year 1880 was coupled with literature data, providing a comprehensive basis for the calculation of regional historical nutrient emissions into rivers, river nutrient concentrations, and nutrient loading into the sea. Four different scenarios were calculated: scenario 1 assumed RC following previously established criteria for lakes (Poikane et al. in Environ Manag 45(6):1286–1298, 2010), scenario 4 applied the nutrient emission conditions of 1880 (considering non-intensive land usage, the presence of tile drainage systems, sewer systems, and the human population from 1880), and scenarios 2 and 3 considered intermediate conditions. Our results showed that nutrient emissions from scenario 1 accounted for approximately one-tenth of the currently observed total nitrogen (TN) and total phosphorus (TP) emissions. The nutrient emissions calculated for 1880 (scenario 4) were found to be 47 % (TN) and 55 % (TP) higher than those calculated considering reference lake conditions (scenario 1). Our results suggest that RC nutrient concentrations in rivers in the German Baltic Sea catchment were clearly below 0.05 mg/l for TP and 1 mg/l for TN. This range is in accordance with historical and calculated pristine nutrient concentrations from other rivers in similar catchments.     

Stakeholder perceptions of grassland ecosystem services in relation to knowledge on soil fertility and biodiversity

The concept of ecosystem services is increasingly being used by scientists and policy makers. However, most studies in this area have focussed on factors that regulate ecosystem functions (i.e. the potential to deliver ecosystem services) or the supply of ecosystem services. In contrast, demand for ecosystem services (i.e. the needs of beneficiaries) or understanding of the concept and the relative ranking of different ecosystem services by beneficiaries has received limited attention. The aim of this study was to identify in three European mountain regions the ecosystem services of grassland that different stakeholders identify (which ecosystem services for whom), the relative rankings of these ecosystem services, and how stakeholders perceive the provision of these ecosystem services to be related to agricultural activities. We found differences: (1) between farmers?? perceptions of ecosystem services across regions and (2) within regions, between knowledge of ecosystem services gained by regional experts through education and farmers?? local field-based knowledge. Nevertheless, we identified a common set of ecosystem services that were considered important by stakeholders across the three regions, including soil stability, water quantity and quality, forage quality, conservation of botanical diversity, aesthetics and recreation (for regional experts), and forage quantity and aesthetic (for local farmers). We observed two contrasting stakeholder representations of the effects of agricultural management on ecosystem services delivery, one negative and the other positive (considering low to medium management intensity). These representations were determined by stakeholders?? perceptions of the relationships between soil fertility and biodiversity. Overall, differences in perceptions highlighted in this study show that practitioners, policy makers and researchers should be more explicit in their uses of the ecosystem services concept in order to be correctly understood and to foster improved communication among stakeholders.     

The influence of metabolically engineered glucosinolates profiles in Arabidopsis thaliana on Plutella xylostella preference and performance

The oviposition preference and larval performance of the diamondback moth (DBM), Plutella xylostella, was studied using Arabidopsis thaliana plants with modified glucosinolate (GS) profiles containing novel GSs as a result of the introduction of individual CYP79 genes. The insect parameters were determined in a series of bioassays. The GS content of the plants as well as the number of trichomes were measured. Multivariate analysis was used to determine the possible relationships among insect and plant variables. The novel GSs in the tested lines did not appear to have any unequivocal effect on the DBM. Instead, the plant characteristics that affected larval performance and larval preference did not influence oviposition preference. Trichomes did not affect oviposition, but influenced larval parameters negatively. Although the tested A. thaliana lines had earlier been shown to influence disease resistance, in this study no clear results were found for P. xylostella.     

Local authorities and climate protection in the European union: Putting subsidiarity into practice?

Abstract

Although intrinsically a global environmental concern, the climate change issue has a number of implications for local authorities. Within the European Union (EU), the remit of local authorities in—‐for abatement policies—the crucial areas of energy and transport policy varies considerably, as does their commitment to environmental action more generally. This paper examines the role of local authorities in climate protection within a framework of global, EU and national action, through examples of local strategies from Germany, Italy, Spain, Sweden and the UK. The paper finds positive instances of action in all countries, but points to unsympathetic policy frameworks both at national and EU level, such as budget constraints imposed on local authorities, the pursuit of low energy prices combined with a resistance to impose carbon taxes, a lack of energy efficiency standards and insufficient support for public transport.